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| Radonic,Laura Mabel; Zimmermann,Julián Marcos; Zavallo,Diego; López,Nilda; López Bilbao,Marisa. |
| There is evidence that overexpression of transgenes codifying antifungal proteins may confer protection to pathogen attack, and that this protection is increased due to the synergic effect of the expression of two or more genes. On the other hand it is well known that sunflower is a recalcitrant specie, highly difficult to be genetically transformed. In this context, the final aim of this project was to obtain sunflower plants expressing at least two antifungal genes, avoiding sequential transformation. The antifungal genes used encode for two enzymes that degrade the fungal wall (glucanase and chitinase), an osmotin and a ribosome inhibitor protein. Two types of transformation vectors were used: a more traditional system with a double cassette and a novel... |
| Tipo: Journal article |
Palavras-chave: Antifungal genes; Double expression cassettes; Polyprotein cassettes; Sunflower; Transgenic plants. |
| Ano: 2008 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000500008 |
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